Mushroom Growers Handbook 1 Oyster Mushroom Cultivation
The prepared grains are filled into half liter milk bottles or polypropylene bags 150 200g per bottle or bag and autoclaved for 2 hours at 121 After sterilization the material should have a pH value of 7 The bottles are inoculated with grains or bits of agar medium colonized with mycelium and then incubated at 22 24 in a dark place